Letter | Published:

Overnight Incubation Technique for obtaining Transformable Bacillus subtilis Cells of Reproducible Competency

Naturevolume 199pages828829 (1963) | Download Citation



THE use of Bacillus subtilis for DNA-mediated transformation investigations has found wide acceptance since the system was described by Spizizen1 in 1958. In all bacterial transforming systems, technical difficulties arise in having available at the desired time a recipient bacterial population containing a high per cent of transformable (competent) cells. The usual preparation of B. subtilis recipient cells involves overnight incubation in broth, a 4-h period in growth medium followed by a 90-min period in transformation medium before maximum competence is obtained2. These freshly prepared cells are highly competent, although the competence-level will vary and cannot always be predicted.

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  1. 1

    Spizizen, J., Proc. U.S. Nat. Acad. Sci., 44, 1072 (1958).

  2. 2

    Anagnostopoulos, C., and Spizizen, J., J. Bacteriol., 81, 741 (1961).

  3. 3

    Spizizen, J. (personal communication).

  4. 4

    Romig, W. R., Virology, 16, 452 (1962).

  5. 5

    Jensen, R. A., and Haas, F. L., Biochim. Biophys. Acta, 61, 963 (1962).

  6. 6

    Saito, H., Kohiyama, M., and Ideda, Y., J. Gen. App. Microbiol., 7, 243 (1961).

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  1. Palo Alto Medical Research Foundation, 860 Bryant Street, Palo Alto, Calif.



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