Letter | Published:

Localization and Fractional Determination of Neutral Deoxyribonuclease Activity in Cat Serum

Naturevolume 199pages697699 (1963) | Download Citation



THE biological role, biochemical and enzymatic characteristics and cellular localization of DNases in higher mammals are still little understood. The existence of numerous isozymes suggests that the DNase activity manifested by most tissues could be related to one or several molecular forms of one or several enzymes or their inhibitors. In this respect we present a preliminary investigation concerning the localization of DNase activity in the serum of the cat fractionated on gelose and starch gel, and the concentration and fractional determination of this activity by chromatography on substituted celluloses. When compared with various mammalian sera, the cat's serum has a very high DNase activity and therefore constitutes good material for examination1. Blood was obtained by cardiac pimcture from animals anæsthetized with ether. The serum was obtained immediately after retraction of the clot and kept in the refrigerator. It was fractionated by starch-gel electrophoresis according to Smithies2,3 and by agar-gel electrophoresis according to Grabar and Burtin4.

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    Kurnick, N. B., and Carrera, A. E., Proc. Soc. Exp. Biol. Med., 84, 618 (1953).

  2. 2

    Smithies, O., Biochem. J., 61, 629 (1955).

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    Poulik, M. D., and Smithies, O., Biochem. J., 68, 636 (1958).

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    Grabar, P., and Burtin, P., Analyse Immunoelectrophoretique (Masson et Cie., 1960).

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    Sober, H. A., Gutter, F. J., Wyckoff, M., and Peterson, E. A., J. Amer. Chem. Soc., 78, 756 (1956).

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    Le Talaer, J. Y., Festy, B., and Truhaut, R., Clin. Chim. Acta (in the press).

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    Kurnick, N. B., Arch. Biochem., 29, 41 (1950).

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    Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randell, R. J., J. Biol. Chem., 193, 265 (1961).

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    Paoletti, C., Auger, P., Gosse, C., and Truhaut, R. (in preparation).

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  1. Unité de Biochimie, Institut G. Roussy, Villejuif, France

    • G. RIOU
    •  & C. PAOLETTI


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