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Chromatographic Separation of Hæmoglobin and Myoglobin on ‘Sephadex’ Gel

Nature volume 198, pages 1201–1202 (1963)Cite this article

Abstract

THE separation of bovine carboxyhæmoglobin and whale carboxymyoglobin by ion exchange chromatography on DEAE-cellulose has been described by Brown1. Recently, Flodin2 has discussed the fractionation of some peptides and proteins utilizing the molecular sieve action of cross-linked dextran gels (‘Sephadex’). In the course of our work on the chromatography and microheterogeneity of myoglobin, we have found that oxyhæmoglobin and ferrimyoglobin can be easily separated on ‘Sephadex G-75’ and ‘Sephadex G-50’ columns.

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References

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Authors and Affiliations

  1. Department of Chemistry, American University of Beirut, Beirut, Lebanon

    ELIAS AWAD, BRUCE CAMERON & LEILA KOTITE

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  1. ELIAS AWAD
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  2. BRUCE CAMERON
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  3. LEILA KOTITE
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AWAD, E., CAMERON, B. & KOTITE, L. Chromatographic Separation of Hæmoglobin and Myoglobin on ‘Sephadex’ Gel. Nature 198, 1201–1202 (1963). https://doi.org/10.1038/1981201a0

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