Abstract
IN previous publications some kinetic investigations concerning the optimal physico-chemical conditions of incorporation of heterologous and homologous DNA into Novikoff hepatoma cells and Ehrlich ascites carcinoma cells have been given1,2. The first phase of incorporation of DNA process into somatic cells is an adsorption of donor DNA molecules on to the cell surface. This adsorption process is not dependent on time and temperature in the Novikoff hepatoma cells. The amount of radioactive DNA is the same at 0° C and 37° C, but is significantly dependent on the kind and composition of the incubation medium (optimum: Robinson's medium and 1/15 M phosphate buffer, pH 6.5, v/v).
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WILCZOK, T. Influence of Molecular Weight of Deoxyribonucleic Acid on its Incorporation into Neoplastic Cells. Nature 196, 1314–1315 (1962). https://doi.org/10.1038/1961314a0
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DOI: https://doi.org/10.1038/1961314a0
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