Abstract
IN order to determine true relative retentions or partition coefficients in gas-liquid chromatography the ‘gas hold-up’1 of the apparatus must be subtracted from all retention data measured from the point of injection. It has been recommended1 that the time or gas volume between the injection point and the emergence of the air peak should be taken as the gas hold-up. Moreover, when using detectors which do not respond to atmospheric gases the use of methane has been suggested2.
Similar content being viewed by others
Article PDF
References
Ambrose, D. A., James, A. T., Keulemans, A. I. M., Kovats, E., Röck, H., Rouit, C., and Stross, F. H., Pure and App. Chem., 1, 177 (1960).
Feinland, R., Ardreatch, A. J., and Cotrupe, D. P., Anal. Chem., 33, 991 (1961).
Smith, J. F., Chem. and Indust., 1024 (1960).
Evans, M. B., and Smith, J. F., J. Chromatog., 6, 293 (1961).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
SMITH, J. Non-Coincidence of ‘Gas Hold-Up’ and Retention of Air in Gas-Liquid Chromatography. Nature 193, 679 (1962). https://doi.org/10.1038/193679a0
Issue Date:
DOI: https://doi.org/10.1038/193679a0
This article is cited by
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.