Letter | Published:

Identification of the Main Ethylenediamine Condensate of Noradrenaline with that of Catechol

Nature volume 193, pages 484485 (03 February 1962) | Download Citation

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Abstract

BECAUSE of the importance of fluorometric microanalysis of catecholamines, the ethylenediamine condensation method1 should be examined in detail. The specificity of this method for noradrenaline might be important. In previous communications2,3, we indicated that the main condensation product of noradrenaline or of 3,4-dihydroxymandelic acid is considered to be the same as that of catechol, and that the side-chain of noradrenaline or of 3,4-dihydroxymandelic acid might be damaged severely during the condensation reaction. Weil-Malherbe4 reported that with catechol, noradrenaline and 3,4-dihydroxymandelic acid, two main products which appear to be identical for the three compounds are formed. According to Joly and Nadeau5, at least three of the fluorescent spots on a two-dimensional paper chromatogram of an ethylenediamine condensate of tagged adrenaline (β-14C-DL-adrenaline) were not radioactive, and these three fluorescent spots were found to be common to the three chromatograms obtained from adrenaline, noradrenaline and catechol.

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Affiliations

  1. Department of Biochemistry, School of Medicine, Nagoya University, Nagoya.

    • TOSHIHARU NAGATSU
    •  & KUNIO YAGI

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https://doi.org/10.1038/193484a0

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