Abstract
COLLAGEN contains a very small amount of histidine in the order of 2 : 419 residues of amino-acids1. Hæmoglobin of red cells has as much as 8 per cent histidine2. Autoradiography with tritiated histidine appears to offer an adequate means of examining this amino-acid in the skeletal system in spite of its low concentration. Such an investigation is of interest from several aspects, namely: (1) an adequate and specific histochemical method for histidine is lacking; (2) it would be of basic significance to ascertain whether intracellular histidine is utilized in bone and cartilage matrix production.
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References
Gustavson, K. H., The Chemistry and Reactivity of Collagen, 39 (Academic Press, New York, 1956).
Pearse, A. G. E., Histochemistry Theoretical and Applied, second ed., 128 (Little, Brown and Co., Boston, 1960).
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Gould, B. S., Manner, G., Goldman, H. M., and Stolman, J. M., Ann. N.Y. Acad. Sci., 85, 385 (1960).
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TONNA, E. An Autoradiographic Examination of the Utilization of Tritiated Histidine by Cells of the Skeletal System. Nature 193, 1301–1302 (1962). https://doi.org/10.1038/1931301a0
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DOI: https://doi.org/10.1038/1931301a0
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