Abstract
IT is well known that the application of long-chain fatty acid samples to gas chromatography needs their previous esterification to methyl esters. Speaking generally, there are two ways to obtain such esters: (1) The esterification of the fatty acids with diazomethane, or methanol–hydrochloric acid, after saponification of the lipids and removal of the non-saponifiable compounds. (2) The direct transfer of the fatty acids in ester combination from glycerol to methanol1.
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References
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RUGGIERI, S. Separation of the Methyl Esters of Fatty Acids by Thin Layer Chromatography. Nature 193, 1282–1283 (1962). https://doi.org/10.1038/1931282a0
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DOI: https://doi.org/10.1038/1931282a0
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