Abstract
SINCE 1956, increased attention has been given to techniques for freeze-drying bovine spermatozoa as a method for long-term semen preservation1–5. In one approach1–3, spermatozoa were dried in vacuum after being diluted, glyeerolated, equilibrated and slowly frozen. Low survival-rates and frequent failures were encountered. Leidl3 suggested three possible causes: (a) concentration of glycerol to toxic levels; (b) excessive removal of water; (c) morphological damage (occasional broken mid-pieces).
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References
Albright, J. L., Erb, R. E., and Ehlers, M. H., J. Dairy Sci., 41, 206 (1958).
Bialy, G., and Smith, V. R., J. Dairy Sci., 40, 739 (1957).
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SAACKE, R., ALMQUIST, J. Freeze-drying of Bovine Spermatozoa. Nature 192, 995–996 (1961). https://doi.org/10.1038/192995a0
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DOI: https://doi.org/10.1038/192995a0
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