Letter | Published:

Freeze-drying of Bovine Spermatozoa

Naturevolume 192pages995996 (1961) | Download Citation

Subjects

Abstract

SINCE 1956, increased attention has been given to techniques for freeze-drying bovine spermatozoa as a method for long-term semen preservation1–5. In one approach1–3, spermatozoa were dried in vacuum after being diluted, glyeerolated, equilibrated and slowly frozen. Low survival-rates and frequent failures were encountered. Leidl3 suggested three possible causes: (a) concentration of glycerol to toxic levels; (b) excessive removal of water; (c) morphological damage (occasional broken mid-pieces).

Access optionsAccess options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1

    Albright, J. L., Erb, R. E., and Ehlers, M. H., J. Dairy Sci., 41, 206 (1958).

  2. 2

    Bialy, G., and Smith, V. R., J. Dairy Sci., 40, 739 (1957).

  3. 3

    Leidl, W., Proc. Third Intern, Congr. Animal Reprod., Cambridge, 3, 39 (1956).

  4. 4

    Meryman, H. T., and Kafig, E., Nature, 184, 470 (1959).

  5. 5

    Yushchenko, N. P., Proc. Lenin. Acad. Agric. Sci., 22 (6), 37 (1957).

  6. 6

    Caufield, J. B., J. Biophys. Biochem. Cytol., 3, 827 (1957).

  7. 7

    Palade, G. E., J. Exp. Med., 95, 285 (1952).

Download references

Author information

Affiliations

  1. Dairy Breeding Research Center, Department of Dairy Science, Pennsylvania State University, University Park

    • R. G. SAACKE
    •  & J. O. ALMQUIST

Authors

  1. Search for R. G. SAACKE in:

  2. Search for J. O. ALMQUIST in:

About this article

Publication history

Issue Date

DOI

https://doi.org/10.1038/192995a0

Further reading

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.