Letter | Published:

Determination of the Amino-Acid Composition of Bovine Growth Hormone

Naturevolume 192pages971972 (1961) | Download Citation



THE amino-acid composition of bovine growth hormone has been determined1 by Levy's method2. This procedure, which involves paper chromatography of dinitrophenyl derivatives of amino-acids, is subject to certain limitations such as variable recoveries of tyrosine and lack of resolution of the leucine derivatives. The development of automated ion-exchange chromatography of amino-acids3 has prompted a re-investigation of the amino-acid composition of this protein.

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    Li, C. H., and Chung, D., J. Biol. Chem., 218, 33 (1956).

  2. 2

    Levy, A. L., Nature, 174, 126 (1954).

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    Spackman, D. H., Stein, W. H., and Moore, S., Anal. Chem., 30, 1190 (1958).

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    Li, C. H., J. Biol. Chem., 211, 555 (1954).

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    Ferguson, K. A., and Wallace, A. L. C., Nature, 190, 629 (1961).

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    Léonis, J., and Li, C. H., Biochim. Biophys. Acta, 28, 520 (1958).

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  1. Department of Biochemistry, The Upjohn Co., Kalamazoo, Michigan



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