Letter | Published:

Determination of the Amino-Acid Composition of Bovine Growth Hormone

Nature volume 192, pages 971972 (09 December 1961) | Download Citation

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Abstract

THE amino-acid composition of bovine growth hormone has been determined1 by Levy's method2. This procedure, which involves paper chromatography of dinitrophenyl derivatives of amino-acids, is subject to certain limitations such as variable recoveries of tyrosine and lack of resolution of the leucine derivatives. The development of automated ion-exchange chromatography of amino-acids3 has prompted a re-investigation of the amino-acid composition of this protein.

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Affiliations

  1. Department of Biochemistry, The Upjohn Co., Kalamazoo, Michigan.

    • ALAN J. PARCELLS

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DOI

https://doi.org/10.1038/192971a0

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