Letter | Published:

Influence of pH and Urea on the Ultra-violet Fluorescence of Several Globular Proteins

Nature volume 192, pages 873874 (02 December 1961) | Download Citation

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Abstract

RECENT work by Weber1–3 has shown that the ultraviolet fluorescent spectra of globular proteins differ from those predicted for the summation of the independent contributions of their aromatic amino-acid constituents. Those examples investigated which contained tyrosine, but no tryptophan, showed a tyrosine emission spectrum, but with a reduced quantum yield. Those containing both tyrosine and tryptophan showed an emission spectrum predominantly resembling that of tryptophan alone, with an emission maximum in the range 320–350 mµ. The present communication will be concerned solely with proteins of the second category.

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Author information

Affiliations

  1. Naval Medical Research Institute, National Naval Medical Center,

    • R. F. STEINER
  2. National Institute of Arthritis and Metabolic Disease, National Institutes of Health, Bethesda, Maryland.

    • H. EDELHOCH

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DOI

https://doi.org/10.1038/192873a0

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