Abstract
Coons and Kaplan1 in their description of the fluorescent antibody technique using antisera coupled with fluorescein isocyanate found that the nonspecific staining which occurred when such conjugates were applied to tissue sections could be minimized by dialysing the conjugates against 0.01 M phosphate buffer solution pH 7.0 for several days, followed by treatment with liver powder.
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References
Coons, A. H., and Kaplan, M. H., J. Exp. Med., 91, 1 (1950).
Marshall, J. D., Eveland, W. C., and Smith, C. W., Proc. Soc. Exp. Biol., 98, 898 (1958).
Glynn, L. E., Holborow, E. J., and Johnson, G. D., Lancet, ii, 1083 (1957).
Holborow, E. J., Weir, D. M., and Johnson, G. D., Brit. Med. J., ii, 732 (1957).
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JOHNSON, G. Simplified Procedure for removing Non-specific Staining Components from Fluorescein-labelled Conjugates. Nature 191, 70–71 (1961). https://doi.org/10.1038/191070a0
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DOI: https://doi.org/10.1038/191070a0
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