Letter | Published:

Separation Methods based on Molecular Sieving and Ion Exclusion

Naturevolume 191pages6970 (1961) | Download Citation

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Abstract

CROSS-LINKED dextran (‘Sephadex’) has been shown to possess properties of a molecular sieve1. The size limits for effective molecular sieving depend on the degree of cross-linking. Molecules or ions capable of entering the gel phase may be subject to specific interaction with the matrix substance. Thus, for example, when certain aromatic compounds are passed through ‘Sephadex’ columns, a more extensive retardation is found than if the gel acted as a molecular sieve only.

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References

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    Porath, J., and Flodin, P., Nature, 183, 1657 (1959); Biochim. Biophys. Acta, 39, 193 (1960). Gelotte, B., J. Chromatography, 3, 330 (1960). Flodin, P., ibid., 5, 103 (1961).

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Affiliations

  1. Institute of Biochemistry, University of Uppsala

    • J. PORATH
    •  & E. B. LINDNER

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https://doi.org/10.1038/191069a0

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