Letter | Published:

Separation Methods based on Molecular Sieving and Ion Exclusion

Naturevolume 191pages6970 (1961) | Download Citation



CROSS-LINKED dextran (‘Sephadex’) has been shown to possess properties of a molecular sieve1. The size limits for effective molecular sieving depend on the degree of cross-linking. Molecules or ions capable of entering the gel phase may be subject to specific interaction with the matrix substance. Thus, for example, when certain aromatic compounds are passed through ‘Sephadex’ columns, a more extensive retardation is found than if the gel acted as a molecular sieve only.

Access optionsAccess options

Rent or Buy article

Get time limited or full article access on ReadCube.


All prices are NET prices.


  1. 1

    Porath, J., and Flodin, P., Nature, 183, 1657 (1959); Biochim. Biophys. Acta, 39, 193 (1960). Gelotte, B., J. Chromatography, 3, 330 (1960). Flodin, P., ibid., 5, 103 (1961).

  2. 2

    Lindner, E. B., Elmqvist, A., and Porath, J., Nature, 184, 1565 (1959).

  3. 3

    Moore, S., and Stein, W. H., J. Biol. Chem., 211, 907 (1954).

  4. 4

    Wheaton, R. M., and Bauman, W. C., Indust. Eng. Chem., 45, 228 (1953).

  5. 5

    Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J., J. Biol. Chem., 193, 265 (1951).

Download references

Author information


  1. Institute of Biochemistry, University of Uppsala

    • J. PORATH
    •  & E. B. LINDNER


  1. Search for J. PORATH in:

  2. Search for E. B. LINDNER in:

About this article

Publication history

Issue Date



Further reading


By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.