Preparation of Sheep Growth Hormone

Abstract

EXISTING methods for the preparation of sheep growth hormone by low-temperature ethanol fractionation1 or salt fractionation, combined with ion exchange chromatography2, are lengthy and involve a large number of individual steps. The same criticism applies to existing methods for preparation of ox growth hormone3,4.

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References

  1. 1

    Wilhelmi, A. E., Hypophysial Growth Hormone—Nature and Actions, edit. by Smith, Gaebler and Long (MeGraw-Hill, 1954).

  2. 2

    Papkoff, H., and Li, C. H., Biochim. Biophys. Acta, 29, 145 (1958).

  3. 3

    Li, C. H., Evans, H. M., and Simpson, M. E., J. Biol. Chem., 159, 353 (1945).

  4. 4

    Wilhelmi, A. E., Fishman, J. B., and Russell, J. A., J. Biol. Chem., 176, 735 (1948).

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WALLACE, A. Preparation of Sheep Growth Hormone. Nature 190, 535–536 (1961). https://doi.org/10.1038/190535a0

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