Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Letter
  • Published:

Influence of Oxygen Concentration and Mechanical Factors on Differentiation of Connective Tissues in vitro

Nature volume 190, pages 460–461 (1961)Cite this article

Abstract

MARCHAND1 postulated that osteoblasts may differentiate from mesenchymal cells. Recently, it was observed that a strain of cells, derived from adult skeletal muscle, elaborated in tissue culture a sub-stance that appeared to be chondro-osteoid2. Since the factors responsible for this cell behaviour were unknown, investigation of the effects of varying mechanical and nutritional factors on a reproducible in vitro system of osteogenesis was made. Hanging-drop, Maximov double cover-slip cultures were established from 20-day-old chick embryo tibial cortex and were maintained as described by Fell3.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Marchand, F., “Der Prozess der Wundheilung”, in Deutsche Chirurgie, 16, 1 (F. Enke, Stuttgart, 1901).

    Google Scholar 

  2. Bassett, C. A. L., J. Bone and Joint Surg., 41, A, 773 (1959).

    Google Scholar 

  3. Fell, H. B., J. Anat., 66, 157 (1932).

    CAS  PubMed  PubMed Central  Google Scholar 

  4. Fitton-Jackson, S., Randall, J. T., and Ciba Foundation Symp. Bone Structure and Metabolism, edit. by Wolstenholme, G. E. W., and O'Connor, C. M., 57 (Little, Brown and Co., Boston, 1956).

  5. Robinson, R. A., and Watson, M. L., Ann. N.Y. Acad. Sci., 60, 596 (1955).

    Article  ADS  CAS  Google Scholar 

  6. Maximov, A. A., in von Mollendorff, M., Handbuch der mikroskopischen Anatomie des Menschen, 2, Part 1, 232 (J. Springer, Berlin, 1927).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Orthopaedic Research Laboratories, Columbia University, College of Physicians and Surgeons, New York

    C. ANDREW L. BASSETT & INGEBORG HERRMANN

Authors
  1. C. ANDREW L. BASSETT
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. INGEBORG HERRMANN
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

BASSETT, C., HERRMANN, I. Influence of Oxygen Concentration and Mechanical Factors on Differentiation of Connective Tissues in vitro . Nature 190, 460–461 (1961). https://doi.org/10.1038/190460a0

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1038/190460a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing