Use of a ‘Mixed Hæmadsorption’ Method in Virus-infected Tissue Cultures

Abstract

THE principle of mixed agglutination¹ has been used by Högman² to indicate the presence of blood-group antigens in tissue culture. In this system the cultured cells are sensitized with antibodies directed towards antigens of the cell surface. Thereby the cells acquire capacity to adsorb homologous red cells. Vogel and Shelokov³ have shown that certain viruses can be located in tissue cultures by their capacity to adsorb sensitive erythrocytes, the so-called hæmadsorption test. Two pre-requisites for this system are : (1) that virus antigen be available on the surface of the infected tissue cells; (2) that this antigen fix the erythrocytes.