Reduced Folic Acid Analogues as Antimetabolites


HYDROGENATION of the folic acid analogues aminopterin (4-aminopteroylglutamic acid) and amethopterin (N10-methyl-4-aminopteroylglutamic acid) is reported to result in diminished antibacterial activity1,2 against Streptococcus faecalis R (A.T.C.C. 8043) and Pediococcus cerevisiae (A.T.C.C. 8081). In view of the lability of tetrahydrofolic acid it seemed desirable to repeat these experiments taking precautions to avoid destruction of the reduced analogues ; hence they were added to the assay medium in a potassium ascorbate solution after autoclaving3. The ascorbate did not greatly affect the growth of either organism. The media employed were similar to those described4,5

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KISLIUK, R. Reduced Folic Acid Analogues as Antimetabolites. Nature 188, 584–585 (1960).

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