Abstract
THE preparation of dihydropteroylglutamic acid has previously been described by O'Dell et al., who used catalytic hydrogenation of pteroylglutamate1, and by Futterman, who carried out the reduction with sodium dithionite2. The acid prepared by catalytic hydrogenation is unsuitable for many biochemical studies, however, because it contains traces of tetra-hydropteroylglutamic acid3. The material obtained by the method of Futterman is an amorphous discoloured preparation2, but was found to be uncontaminated by tetrahydropteroylglutamic acid3. No analytical figures have been given for this material. By reducing purified pteroylglutamate with sodium dithionite according to the method of Futterman and twice precipitating the product by slow adjustment of the pH. between strictly defined values, it has now been found possible to obtain dihydropteroylglutamic acid consistently as a white crystalline product giving analytical results agreeing fairly well with the theoretical values.
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References
O'Dell, B. L., Vandenbelt, J. M., Bloom, E. S., and Pfiffner, J. J., J. Amer. Chem. Soc., 69, 250 (1947).
Futterman, S., J. Biol. Chem., 228, 1031 (1957).
Blakley, R. L., Biochem. J., 72, 707 (1959).
Blakley, R. L., Biochem. J., 65, 331 (1957).
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BLAKLEY, R. Crystalline Dihydropteroylglutamic Acid. Nature 188, 231–232 (1960). https://doi.org/10.1038/188231a0
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DOI: https://doi.org/10.1038/188231a0
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