Abstract
SHIFTS in the ultra-violet absorption spectra of aqueous organic solutions of some amino-azo dyes on the addition of buffered protein solutions have frequently been interpreted as evidence of complex formation between protein and dye1–4. The effect of the buffer itself on the spectrum of the dye has apparently not always been checked, and the fact that this may influence the results is shown by the following experiments.
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FRENCH, C., PRITCHARD, R. Use of Spectrophotometric Evidence for Protein-Dye Binding. Nature 188, 228 (1960). https://doi.org/10.1038/188228a0
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DOI: https://doi.org/10.1038/188228a0
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