The Ouchterlony Technique using Antigen or Antiserum labelled with Iodine-131

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THE Ouchterlony agar plate technique1 has been used a great deal for the analysis of antibody–antigen systems, but it was not until recently that it was used quantitatively2. An autoradiographic agar plate technique employing radioactive proteins has been described by Perlmann and Hultin3 for the demonstration of proteins in complex systems, but their method was not in any way quantitative.

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  1. 1

    Ouchterlony, O., Acta Path. Microbiol. Scand., 25, 186 (1948).

  2. 2

    Wright, S. T. C., Nature, 183, 1282 (1959).

  3. 3

    Perlmann, P., and Hultin, T., Nature, 182, 1530 (1958).

  4. 4

    MeFarlane, A. S., Nature, 182, 53 (1958).

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RHODES, J. The Ouchterlony Technique using Antigen or Antiserum labelled with Iodine-131. Nature 187, 793–794 (1960) doi:10.1038/187793a0

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