Evidence for Multiple Nature of Cellulase of Myrothecium verrucaria

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CONFLICTING views are held on the multiple nature of cellulase of M. verrucaria. Paper and column chromatography1,2, zone electrophoresis3 and electrophoresis-convection4 of crude preparations of the cellulase indicated the multiple nature of this enzyme system. On the other hand, by fractionating the cellulase by precipitation techniques, Whitaker5 obtained a preparation homogeneous in the ultra centrifuge and on electrophoresis, which exhibited all the activities tested of the crude enzyme concentrated in about the same ratios. He maintains that 1,4-β-linked oligo- and poly-glucosides are hydrolysed by one enzyme6 which possibly forms stable complexes with metabolic products, thus giving rise to a number of components with cellulolytic activity6,7.

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    Reese, E. T., and Gilligan, W., Arch. Biochem. Biophys., 45, 74 (1953).

  2. 2

    Gilligan, W., and Reese, E. T., Canad. J. Microbiol., 1, 90 (1954).

  3. 3

    Miller, G. L., and Blum, R., J. Biol. Chem., 218, 131 (1956).

  4. 4

    Grimes, R. M., Duncan, C. W., and Hoppert, C. A., Arch. Biochem. Biophys., 68, 412 (1957).

  5. 5

    Whitaker, D. R., Arch. Biochem. Biophys., 43, 253 (1953).

  6. 6

    Thomas, R., and Whitaker, D. R., Nature, 181, 715 (1958).

  7. 7

    Reinouts van Haga, P., Nature, 182, 1232 (1958).

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PRINS-VAN DER MEULEN, P., SCHURINGA, G. Evidence for Multiple Nature of Cellulase of Myrothecium verrucaria . Nature 187, 695–696 (1960) doi:10.1038/187695a0

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