Evidence for Multiple Nature of Cellulase of Myrothecium verrucaria

Article metrics

Abstract

CONFLICTING views are held on the multiple nature of cellulase of M. verrucaria. Paper and column chromatography1,2, zone electrophoresis3 and electrophoresis-convection4 of crude preparations of the cellulase indicated the multiple nature of this enzyme system. On the other hand, by fractionating the cellulase by precipitation techniques, Whitaker5 obtained a preparation homogeneous in the ultra centrifuge and on electrophoresis, which exhibited all the activities tested of the crude enzyme concentrated in about the same ratios. He maintains that 1,4-β-linked oligo- and poly-glucosides are hydrolysed by one enzyme6 which possibly forms stable complexes with metabolic products, thus giving rise to a number of components with cellulolytic activity6,7.

Access options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1

    Reese, E. T., and Gilligan, W., Arch. Biochem. Biophys., 45, 74 (1953).

  2. 2

    Gilligan, W., and Reese, E. T., Canad. J. Microbiol., 1, 90 (1954).

  3. 3

    Miller, G. L., and Blum, R., J. Biol. Chem., 218, 131 (1956).

  4. 4

    Grimes, R. M., Duncan, C. W., and Hoppert, C. A., Arch. Biochem. Biophys., 68, 412 (1957).

  5. 5

    Whitaker, D. R., Arch. Biochem. Biophys., 43, 253 (1953).

  6. 6

    Thomas, R., and Whitaker, D. R., Nature, 181, 715 (1958).

  7. 7

    Reinouts van Haga, P., Nature, 182, 1232 (1958).

Download references

Author information

Rights and permissions

Reprints and Permissions

About this article

Cite this article

PRINS-VAN DER MEULEN, P., SCHURINGA, G. Evidence for Multiple Nature of Cellulase of Myrothecium verrucaria . Nature 187, 695–696 (1960) doi:10.1038/187695a0

Download citation

Further reading

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.