Letter | Published:

pH-Chromatography’ of some Amphoteric Antibiotics

Naturevolume 187pages11111112 (1960) | Download Citation



pH-CHROMATOGRAPHY’ is a paper chromatography method which can be used for simultaneous determination of the ionic character of antibiotics and of suitable pH values for their extraction1. The pH-chromatogram of an antibiotic is obtained by chromatographing on a series of strips of chromatographic papers buffered to pH values from 2 to 10. For the development of the strips by the ascending technique an organic solvent saturated with water is used. A suitable solvent is chosen by determining the solubility of the antibiotic being studied by paper chromatography2. In our reports on the pH-chromatography of antibiotics1,3 we described three types of pH-chromatograms, depending on the ionic character of antibiotics: (1) R F values of pH-chromatograms of antibiotics of acidic nature give S-shaped curves, the maxima of which lie in the acidic range ; with increasing pH values the R F values diminish. (2) The R F values of pH-chromatograms of antibiotics of basic character give S-shaped curves of a reverse character ; the maximum of the curves being in the basic and the minimum in the acidic range, respectively. (3) pH-chromatograms of neutral antibiotics have practically the same R F values along the whole range of pH values, giving a straight line parallel with the starting points.

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  1. 1

    Betina, V., Nature, 182, 796 (1958).

  2. 2

    Ševčík, V., Podojil, M., and Vrtišková, A., Csl. mikrobiol., 2, 175 (1957).

  3. 3

    Betina, V., Chemické zvesti, 13, 51 (1959).

  4. 4

    Harned, R. L., Hidy, P. H., and LaBaw, E. K., Antibiotics and Chemotherapy, 5, 204 (1955).

  5. 5

    Schull, G. M., and Sardinas, J. L., Antibiotics and Chemotherapy, 5, 398 (1955).

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  1. Department of Technical Microbiology and Biochemistry, Chemical Faculty of the Slovak Polytechnical University, Bratislava

    •  & PAVEL NEMEC


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