Abstract
AN antiserum against human globin was raised in a rabbit by immunization with globin prepared from adult hæmoglobin by the methods of Anson and Mirsky1 and Jope, Jope and O'Brien2. A 1 per cent solution was injected subcutaneously in Freund's adjuvant3, and intravenously. The resulting antiserum precipitated both human globin and human hæmoglobin; 1 ml. precipitated about 0.5 mgm. of hæmoglobin.
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References
Anson, M. L., and Mirsky, A. E., J. Gen. Physiology, 13, 469 (1930).
Jope, E. M., Jope, H. N., and O'Brien, J. R. P., Nature, 164, 622. (1949).
Freund, J., and McDermott, K., Proc. Soc. Exp. Biol., N.Y., 49, 548 (1942).
Boivin, P., and Hartmann, L., Rev. franç. et Clin. Bioç., 3, 48 (1958).
Allison, A. C., and Humphrey, J. H., Nature, 183, 1590 (1959).
Gutter, F. J., Sober, H. A., and Peterson, E. A., Arch. Biochem. Biophys., 62, 427 (1956).
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ROWE, D., SOOTHILL, J. Observations on Globin, Hæmoglobin and Haptoglobin using a Rabbit Antiglobin Serum. Nature 186, 975–976 (1960). https://doi.org/10.1038/186975a0
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DOI: https://doi.org/10.1038/186975a0
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