Abstract
THE use of bathophenanthroline (4:7-diphenyl-l:10-phenanthroline) as a highly sensitive reagent for the colorimetric estimation of iron has been described by Smith, McCurdy and Diehl1. They showed that it was almost twice as sensitive as 1:10-phenanthroline, that the ferrous-batholphenanthroline complex could be extracted into a suitable solvent, and that it was virtually specific for iron (cobalt formed a yellow non-extractable complex; copper formed a yellow extractable complex, but only in neutral or alkaline solution). In view of these considerations it was decided to use this reagent to replace 1:10-phenanthroline in the estimation of iron in culture medium, and the following method was developed.
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References
Smith, G. F., McCurdy, W. H., and Diehl, H., Analyst, 77, 418 (1952).
Jones, W. A., Biochem. J., 43, 429 (1948).
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SEAMER, P. Estimation of µgm. Quantities of Iron in Culture Medium, using Bathophenanthroline. Nature 184, 636–637 (1959). https://doi.org/10.1038/184636a0
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DOI: https://doi.org/10.1038/184636a0
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