Changes in Fluorescence in a Frog Sartorius Muscle Following a Twitch

Article metrics


IN a recent publication1 it was proposed that the blue autofluorescence of living tissues may largely be due to the reduced pyridine nucleotide of the mitochondria. An opportunity to test this hypothesis is afforded by the specific response of the intramitochondrial pyridine nucleotide to an increase of concentration of adenosine diphosphate following a muscle twitch. This increase of concentration of adenosine diphosphate has been shown by spectro-photometric observations to cause an oxidation of reduced pyridine nucleotide of the mitochondria both in the isolated mitochondria2 and intact muscles3. Thus muscle contraction elicits a specific response of intramitochondrial pyridine nucleotide. Such a specific test is required to distinguish between the fluorescence of cytoplasmic and mitochondrial pyridine nucleotides because both may be bound forms and therefore have indistinguishable maxima at 443 mµ4.

Access optionsAccess options

Rent or Buy article

Get time limited or full article access on ReadCube.


All prices are NET prices.


  1. 1

    Chance, B., and Baltscheffsky, H., J. Biol. Chem., 233, 736 (1958).

  2. 2

    Chance, B., and Williams, G. R., J. Biol. Chem., 217, 409 (1955).

  3. 3

    Chance, B., and Connelly, C. M., Nature, 179, 1236 (1957).

  4. 4

    Duysens, L. M. N., and Amesz, J., Biochim. Biophys. Acta, 24, 19 (1957).

  5. 5

    Theorell, H., and Nygaard, A. P., Acta Chem. Scand., 8, 877 (1954).

  6. 6

    Chance, B., Conrad, H., and Legallais, V., Program and Abstracts of Biophysical Society, Massachusetts Inst. Tech., 44 (Cambridge, 1958).

  7. 7

    Chance, B., Science, 120, 767 (1954).

  8. 8

    Connelly, C. M., and Chance, B., Fed. Proc., 13, Abstr. No. 94 (1954)

Download references

Author information

Rights and permissions

Reprints and Permissions

About this article

Further reading


By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.