Abstract
IN previous experiments on the aerobic degradation of D-xylose by living cells of Torulopsis utilis, we succeeded in isolating the triose-phosphate, pyruvic acid and the acetyl groups using the phenylhydrazine trapping technique. Our results have been confirmed by Heath et al. 3, who purified from Lactobacillus pentosus an enzyme which phosphorolytically cleaves D-xylulose-5-phosphate into triose-phosphate and acetyl-phosphate. Schramm and Racker4 have shown in a mutant of Acetobacter xylinum the presence of an enzyme which carries out the same phosphorolytic split of D-xylulose-5-phosphate and cleaves also the fructose-6-phosphate into erythrose-4-phosphate and acetyl-phosphate. From the results of our preceding experiments we supposed a split of an intermediate phosphorylated ketopentose into triose-phosphate and into an unknown C-2 intermediate, both originating the acetyl group according to the formulation given in ref. 2.
Similar content being viewed by others
Article PDF
References
Bolcato, V., Feruglio, C., and Parigi, G., Il Farmaco, 5, 251 (1950).
Bolcato, V., Scevola, M. E., and Feruglio, G., Arch. Biochem. Biophys., 43, 143 (1953).
Heath, E. C., Hurwitz, J., and Horecker, B. L., J. Amer. Chem. Soc., 78, 5449 (1956).
Schramm, M., Klybas, V., and Racker, E., J. Biol. Chem., 233, 1283 (1958).
Kaushal, R., Jowett, P., and Walker, T. K., Nature, 167, 949 (1951).
Bolcato, V., and Leggiero, G., Experientia (1 15, 388 (1959).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
BOLCATO, V., LEGGIERO, G. Glycolaldehyde Trapped from Aerobic Oxidation of D-Xylose by Torulopsis utilis . Nature 184, 1318 (1959). https://doi.org/10.1038/1841318a0
Issue Date:
DOI: https://doi.org/10.1038/1841318a0
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.