Abstract
THE use of oxidation-reduction colour indicators the solubility of which is unchanged during reduction in the Hill reaction of photosynthesis provides no information as to the site in the chloroplast in which the reaction occurs. Dyar1 used blue tetrazolium, which is colourless and soluble in the oxidized form in water but insoluble and highly coloured when reduced, to localize regions of dehydrogenase activity in plant cells. In chloroplasts reduction was apparently confined to grana-like bodies. In some materials the reaction was dependent on light, suggesting a relation to the Hill reaction. Since non-plastid cytoplasm may have contributed to the reaction, we were interested in whether isolated chloroplasts were capable of reducing the dye.
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References
Dyar, M. T., Amer. J. Bol., 40, 20 (1953).
Jagendorf, A. T., and Evans, M., Plant Physiol., 32, 435 (1957).
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TAYLOR, J., CHESSIN, M. Photochemical Reduction of Blue Tetrazolium by Isolated Chloroplasts. Nature 183, 626 (1959). https://doi.org/10.1038/183626a0
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DOI: https://doi.org/10.1038/183626a0
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