Abstract
THE separation of proteins and enzymes by the ammonium sulphate gradient elution technique has been reported by a number of workers1–4. Dixon5 and Kunitz6 have published a useful nomogram and equation, respectively, for use in the preparation of ammonium sulphate solutions. While the concentration of ammonium sulphate in a solution can be determined by the usual methods (that is, by distillation and titration, by specific-gravity determinations, nesslerization, or by gravimetric methods) they are all time-consuming, often involve large dilutions of the sample and, in general, are not useful for the routine analysis of a large number of samples. The use of refractometry has been investigated in the search for a more rapid method of measuring the concentration of ammonium sulphate in the fractions collected by the gradient elution technique as well as in the classical precipitation method.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Sayre, F. M., Hill, B. R., and Reinhardt, L. G., Proc. Soc. Exp. Biol. Med., 96, 695 (1957).
Zittle, C. A., and Della Monica, E. S., Arch. Biochem. Biophys., 58, 31 (1955).
Schwimmer, S., Nature, 171, 443 (1953).
Zahl, R. K., and Stahl, I., Z. physiol. Chem., 293, 1 (1953).
Dixon, M., Biochem. J., 54, 457 (1953).
Kunitz, M., J. Gen. Physiol., 35, 423 (1952).
Kirk, P. L., “Adv. Protein Chem.”, 3, 154 (1947).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
WHITAKER, J., HUGHES, R. A Rapid Method for determining the Concentration of Ammonium Sulphate Solutions. Nature 183, 603–604 (1959). https://doi.org/10.1038/183603a0
Issue Date:
DOI: https://doi.org/10.1038/183603a0
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.