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Conjugated Forms of Urobilinoid Pigments

Nature volume 183, pages 463–464 (1959)Cite this article

Abstract

TAKASHI NORO1 reported a fraction of urinary urobilinoids not extractable with chloroform but extractable by saponification with potash; this fraction he assumed to be in the ester form of the urobilinoids. We have found that some of the urinary urobilinoids not extractable with chloroform are characterized by their adherence to the gel-like precipitate formed during the extraction. Shaking normal urines, or preferably those from cases of hæmolytic jaundice, with Sevag's chloroform–amyl alcohol (9 : 1) mixture2 results in partition of their urobilinoid contents. After centrifugation, part of the urobilinoids are found bound to the precipitate which collects at the interface and are extractable from it with ethyl acetate but not with chloroform, while the other part passes into the chloroform–amyl alcohol phase.

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Authors and Affiliations

  1. Department of Medicine,

    I. L. KAHÁN

  2. Department of Ophthalmology, University Medical School, Szeged, Hungary

    A. KAHÁN

Authors
  1. I. L. KAHÁN
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  2. A. KAHÁN
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KAHÁN, I., KAHÁN, A. Conjugated Forms of Urobilinoid Pigments. Nature 183, 463–464 (1959). https://doi.org/10.1038/183463c0

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