Ultra-violet Fluorescence of Quinine Sulphate for Detection of Phosphate Ester Spots on Paper

Abstract

A WIDE variety of phosphate esters have great importance as intermediates in biological reactions. Development of adequate procedures for the chromatography and detection of these phosphate esters has, in many respects, lagged behind. With the exception of the method of Runeckles and Krotkov¹, which was developed as a modification of the procedure of Wade and Morgan², the detection of phosphate esters on paper chromatograms has been very laborious and relatively insensitive. Most of the molybdate procedures, in addition to requiring many manipulations, have the disadvantage of employing perchloric acid, which weakens the paper and causes some charring during heating.