Abstract
IN a previous communication1, a method was described for staining the sites of the initiation of calcification in bone and teeth. This method consisted in treating the tissues with hot pyridine for 24 hr. prior to decalcification and then staining with sudan black. Sudan black stained the matrix around the hypertrophic cells in the epiphyseal cartilage just before these cells are removed by capillary erosion.
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References
Irving, J. T., Nature, 181, 704 (1958).
Steenbock, H., and Black, A., J. Biol. Chem., 64, 263 (1925).
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IRVING, J. Histochemical Changes in the Epiphyseal Cartilage during Rickets. Nature 183, 1734–1735 (1959). https://doi.org/10.1038/1831734a0
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DOI: https://doi.org/10.1038/1831734a0
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