Abstract
MARTIUS1 has reported the presence in animal tissues of a quinone reductase (phylloquinone reductase) similar in properties to menadione reductase described by Wosilait and Nason2. Phylloquinone reductase couples the oxidation of reduced diphosphopyridine nucleotide (DPNH) with the reduction of vitamin K3. Since the reduced form of vitamin K3 reduces neotetrazolium chloride (NT) directly to the coloured insoluble formazan3, it is of interest to investigate the production of formazan by the enzymic oxidation of reduced diphosphopyridine nucleotide in order to ascertain if this enzymic sequence involves a quinone reductase. In this communication the enzymic process linking the oxidation of reduced diphosphopyridine nucleotide with reduction of neotetrazolium chloride is termed DPNH–NT reductase.
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References
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Slater, T. F., Nature, 183, 50 (1959).
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SLATER, T. Reduced Diphosphopyridine Nucleotide—Neotetrazolium Reductase. Nature 183, 1679–1680 (1959). https://doi.org/10.1038/1831679a0
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DOI: https://doi.org/10.1038/1831679a0
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