Abstract
METHODS have frequently been described for the detection and estimation of heterocyclic compounds on chromatograms by ultra-violet photography1–4. These methods use a mercury discharge lamp suitably filtered. This type of lamp, however, yields predominantly the 253.7 mµ mercury line and is only suitable for compounds having a considerable absorption at this wave-length. With the benzenoid ring class of compounds, the absorption maxima are generally between 265 mµ and 285 mµ, frequently with the trough near the 253.7 mµ line. Consequently, in order to detect these compounds by a similar technique, a different source of light is necessary.
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MOSS, P., THOMAS, A. Detection and Estimation of Benzenoid Compounds by Chromatography. Nature 183, 1611–1612 (1959). https://doi.org/10.1038/1831611a0
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DOI: https://doi.org/10.1038/1831611a0
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