Abstract
FREQUENTLY when examining autoradiograms of biological specimens there is a need for resolution such as can be obtained with the electron microscope. A technique for preparing autoradiograms for electron microscopy has been reported by Liquier-Milward1. The method described here has the advantage of being somewhat simpler in that nuclear emulsion is applied directly to the specimen grids, and the method is suitable for use with ultra-thin sections. Although only results obtained with yeasts and the alpha-emitter polonium-210 are described, other isotopes and biological specimens can be used.
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References
Liquier-Milward, J., Nature, 177, 619 (1956).
Chapman, G. B., and Hillier, J., J. Bact., 66, 362 (1953).
Agar, H. D., and Douglas, H. C., J. Bact., 70, 427 (1955).
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O'BRIEN, R., GEORGE, L. Preparation of Autoradiograms for Electron Microscopy. Nature 183, 1461–1462 (1959). https://doi.org/10.1038/1831461a0
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DOI: https://doi.org/10.1038/1831461a0
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