Abstract
ACCORDING to Miyazaki, Omachi and Kamata1, antibiotics can be grouped according to their salting-out chromatograms. Pure antibiotics and materials containing antibiotics were examined by the aid of ascending paper chromatography. As irrigation solvents in each case and with each antibiotic, distilled water and rising concentrations of ammonium chloride (0.5, 1, 2, 3, 5, 10, 20 per cent and saturated) were used. Location of the spots of the antibiotics, that is, the R F values, were ascertained bio-autographically.
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References
Miyazaki, J., Omachi, K., and Kamata, T., J. Antibiotics (Japan), 6, 6 (1953).
Úri, J., Bognár, R., Békési, I., and Varga, B., Nature, 182, 401 (1958).
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ÚRI, J. New Types of Salting-out Paper Chromatograms of Antibiotics. Nature 183, 1188–1189 (1959). https://doi.org/10.1038/1831188b0
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DOI: https://doi.org/10.1038/1831188b0
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