Abstract
A MICRO-ELECTROPHORETIC technique has been developed which is sensitive, simple and rapid and does not require any elaborate apparatus. Very clear results can be obtained with samples of the order of fractions of a micro-litre with a protein content of 10 µgm. and even less. The technique is a combination of the membrane filter electrophoresis1 and, in particular, of the small-scale technique2, with the use of a very sensitive stain–‘Nigrosin’3. All details of the membrane-filter electrophoresis apply as well to the micro-technique.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Kohn, J., Clin. Chim. Acta, 2, 297 (1957).
Kohn, J., Clin. Chim. Acta (in the press).
Ortega, M., Nature, 179, 1086 (1957).
Kohn, J., Nature, 180, 986 (1957).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
KOHN, J. A Micro-electrophoretic Method. Nature 181, 839–840 (1958). https://doi.org/10.1038/181839a0
Issue Date:
DOI: https://doi.org/10.1038/181839a0
This article is cited by
-
A rapid micro radial electrophoretic method of protein separation on cellulose acetate membranes
Experientia (1979)
-
Die Mikrozonenelektrophorese des nichteingeengten Liquor cerebrospinalis
Klinische Wochenschrift (1977)
-
Methods for the study of the glycoproteins and proteoglycans of bone using bacterial collagenase
Calcified Tissue Research (1977)
-
An electrophoretic polymorphism in the serum transferrins of Praomys (Mastomys) natalensis
Biochemical Genetics (1976)
-
Ein Beitrag zur Methodik biochemischer Untersuchungen an Hauteiwei�en
Archiv f�r Klinische und Experimentelle Dermatologie (1971)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.