A Possible Intermediate in the Biosynthesis of Tryptophan: I-Deoxy-I-N-o-Carboxyphenyl-Ribulose

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DURING an investigation of compounds formed by washed cell suspensions of mutant strains of Aerobacter aerogenes blocked at various stages in tryptophan synthesis, two strains (N3–33 and N2–18) were found to form a compound which showed an intense blue-violet fluorescence under ultra-violet light, but the absorption spectrum of which did not correspond to any known compound. Neither strain of A. aerogenes would grow in the minimal medium described by Davis and Mingioli1 unless either tryptophan or indole was added and they were therefore presumably unable to convert anthranilic acid to indole.

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  1. 1

    Davis, B. D., and Mingioli, E. S., J. Bact., 60, 17 (1950).

  2. 2

    Gibson, F., Jones, M. J., and Teltscher, H., Biochem. J., 64, 132 (1956).

  3. 3

    Yanofsky, C., J. Biol. Chem., 223, 171 (1956).

  4. 4

    Hodge, J. E., “Adv. Carbohydrate Chem.”, 10, 169 (1955).

  5. 5

    Gibson, F., and Jones, M. J., Aust. J. Sci., 17, 33 (1954).

  6. 6

    Aronoff, S., and Vernon, L., Arch. Biochem., 28, 424 (1950).

  7. 7

    Aronoff, S., “Techniques of Radiobiochemistry” (Iowa State College Press, Ames, 1956).

  8. 8

    Borenfreund, E., and Dische, Z., Arch. Biochem. Biophys., 67, 239 (1957).

  9. 9

    Johanson, R., Nature, 172, 956 (1953).

  10. 10

    Trevelyan, W. E., Procter, D. P., and Harrison, J. S., Nature, 166, 444 (1950).

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GIBSON, F., DOY, C. & SEGALL, S. A Possible Intermediate in the Biosynthesis of Tryptophan: I-Deoxy-I-N-o-Carboxyphenyl-Ribulose. Nature 181, 549–550 (1958) doi:10.1038/181549a0

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