Quantitative Gram-staining with Labelled Iodine

Article metrics

Abstract

THE development of a simple quantitative Gram staining technique has been attempted by numerous observers1,2, not only for its possible practical uses but also because of its potential value in studies on the mechanism of the staining reaction on intact cells and on extracellular material3, as well as the relationship between degree of Gram-positivity and physiological state of the organisms. The recent procedure of Barbaro and Kennedy4 is reasonably accurate but, since it involves measurement of dye nitrogen taken up by a suspension of organisms, it is rather laborious for practical application.

Access options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1

    Bartholomew, J. W., and Mittwer, T., Bact. Rev., 16, 1 (1952).

  2. 2

    Shugar, D., Postepy Hig. Med. Doswiad., 8, 87 (1954).

  3. 3

    Shugar, D., and Baranowska, J., Biochim. Biophys. Acta, 23, 227 (1957).

  4. 4

    Barbaro, J. F., and Kennedy, E. R., J. Bact., 67, 603 (1954).

  5. 5

    Manual of Pure Culture Study: Bacteria” (Soc. Amer. Bact., Biotech. Pub., Geneva, N.Y.).

  6. 6

    Stearn, A. E., and Stearn, E. W., Stain Tech., 5, 17 (1930).

  7. 7

    Hoffman, H., Nature, 168, 464 (1951).

  8. 8

    Zoltman, A. M., and Kahn, M. J., Amer. Chem. Soc., 76, 1554 (1954).

Download references

Author information

Rights and permissions

Reprints and Permissions

About this article

Cite this article

SHUGAR, D., BARANOWSKA, J. Quantitative Gram-staining with Labelled Iodine. Nature 181, 357–358 (1958) doi:10.1038/181357a0

Download citation

Further reading

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.