Abstract
A QUALITATIVE test for the recognition of urine contamination of fabrics has been described by Ishler et al. 1. With the aid of urease the ammonia evolved was detected with Feigl's reagent2. This reagent (silver manganese nitrate solution) appears to be rather more sensitive to ammonia than is Nessler's reagent (alkaline potassium-mercuri-iodide solution), having its lower limit of detection in the order of 0.5 µgm. ammonia. Using Feigl's reagent and a filter paper spot test technique we have devised a rapid and economic method of determining the concentration of urea in 5–20 µl. of biological fluid.
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References
Ishler, N. H., Sloman, K., and Walker, M. E., J. Assoc. Off. Agric. Chem., 30, 670 (1947).
Feigl, F., Mikrochemie, 13, 132 (1933).
Van Slyke, D. D., and Cullen, G. E., J. Biol. Chem., 19, 211 (1914).
Archer, H. E., and Robb, G. D., Quart. J. Med., 18, 274 (1925).
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WATSON, D., PRATT, D. Micro Estimation of Urea. Nature 181, 1475 (1958). https://doi.org/10.1038/1811475a0
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DOI: https://doi.org/10.1038/1811475a0
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