Abstract
THE phosphorylated compounds in leaves of Thatcher wheat plants have been studied after supplying radioactive orthophosphate (phosphorus-32). After killing, extraction of the leaves with boiling alcohol, and evaporation of the extracts to small volume, aliquots were fractionated according to the procedure of Runeckles and Krotkov1, involving ionophoresis and paper chromatography. In addition to several of the commonly occurring phosphate esters, several radioactive unknowns were detected on subsequent autoradiograms. This communication deals with the identification of two of these compounds.
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References
Runeckles, V. C., and Krotkov, G., Arch. Biochem. Biophys., 70, 442 (1957).
Barnard, P. W. C., Bunton, C. A., Llewellyn, D. R., Oldham, K. G., Silver, B. L., and Vernon, C. A., Chem. and Indust., 760 (1955).
Szulmajster, J., Grunberg-Manago, M., and Aubel, E., Bull. Soc. Chim. Biol., 35, 455 (1953).
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RUNECKLES, V. Formation of Alkyl Phosphates in Wheat Leaves. Nature 181, 1470–1471 (1958). https://doi.org/10.1038/1811470a0
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DOI: https://doi.org/10.1038/1811470a0
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