Letter | Published:

Multiplication of Solitary HeLa Cells


THERE are many occasions when it is desirable to grow animal cells from solitary inocula (for example, in order to obtain clones); for this reason, it seems worthwhile to record the following conditions which allow multiplication of isolated HeLa cells.

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    de Fonbrune, P., “Technique de Micromanipulation” (Masson et Cie, Paris, 1949).

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    Lwoff, A., Dulbecco, R., Vogt, M., and Lwoff, M., Virology, 1, 128 (1955).

  3. 3

    Puck, T. T., Ciecuira, S. J., and Fisher, H. W., J. Exp. Med., 106, 145 (1956).

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