Letter | Published:

Maceration of Plant-tissues with Ethylene-diamine-tetra-acetic Acid


FOR many years plant anatomists have used macerating techniques1–3 which subject plant-tissues to either destructive oxidizing agents (usually chromic acid), concentrated acid or alkali, or high temperature. These procedures can cause change in volume of the cells. Macerating media containing chromic acid have been found to destroy cell-walls3 and the other media referred to above would also be very damaging to plant-cells.

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  1. 1

    Tupper-Carey, R. M., and Priestley, J. H., Proc. Roy. Soc., B, 95, 109 (1924).

  2. 2

    McLean, R. C., and Cook, W. R. I., “Plant Science Formulæ”, 85 (Macmillan, London, 1941).

  3. 3

    Brown, R., and Rickless, P., Proc. Roy. Soc., B, 136, 110 (1949).

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