Letter | Published:

Microchemical Method for the Determination of Sakaguchi-positive Antibiotics


ANTIBIOTICS of actinomycete origin containing a guanidine group (such as streptomycin and viomycin) are known to have an antitubercular action. Recently a new antibiotic, primycin, was described1 as an agent active against various mycobacteria and Gram-positive pathogens (0.02 µgm./ml.), and also giving a positive Sakaguchi reaction.

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    Vályi-Nagy, T., Uri, J., and Szilágyi, I., Nature, 174, 1105 (1954).

  2. 2

    Sakaguchi, S., Biochem. J., 5, 25 (1925); Jap. Med. J., 1, 278 (1948).

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    Weber, C. J., J. Biol. Chem., 86, 217 (1930).

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    Kraut, H., Schrader-Beistein, E., and Weber, M., Hoppe-Seyl. Z. phys. Chem., 286, 248 (1951).

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    Ziegler, K., Späth, A., Schaaf, E., Schumann, W., and Winkelmann, E., Liebigs Ann. Chem., 551, 80 (1942).

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