Abstract
THE artificial elimination of F-factor in Escherichia coli K-12 was obtained by treating F+ cells in a cobalt- or nickel-containing medium1. Of the two methods described in that communication, the direct method was difficult to apply to some strains derived from K-12, because the facility of conversion of F+ cells to Fâ cells varied with the strains used. On the other hand, the resistant-isolation method consistently gave Fâ cells, but was somewhat tedious, because the development of resistance to cobalt or nickel was very slow. Recently, however, it has been found that acrinavine is more effective for converting F+ cells into Fâ than cobalt or nickel and other metals (Ikoma, I., unpublished work).
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References
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HIROTA, Y., IIJIMA, T. Acriflavine as an Effective Agent for eliminating F-factor in Escherichia coli K-12. Nature 180, 655â656 (1957). https://doi.org/10.1038/180655a0
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DOI: https://doi.org/10.1038/180655a0
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