Abstract
IT is obviously advantageous to investigate the metabolism of mammalian cells maintained as a suspension in vitro without previous damage to them by mechanical or enzymatic treatment. Circulatory leucocytes are eminently suitable for such a study, the more so because of their role in defence mechanisms. Most of the work done on leucocytes has been concerned with their morphology, the cells being usually derived from bone marrow and from pathological cases; and cultivation has been carried out using some solid or semi-solid supporting medium. We have been able to maintain normal circulatory leucocytes in vitro as a suspension for three to four days using a medium similar to that employed by Osgood et al. 1.
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References
Krippaehne, M. L., and Osgood, E. E., Acta Haematologica, 13, 145 (1955). Osgood, E. E., and Krippaehne, M. L., ibid., 13, 153 (1955).
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MALEC, J., ZAKRZEWSKI, K. Survival of Human Circulatory Leucocytes in vitro: Life-span and Nucleic Acid Turnover. Nature 180, 551–552 (1957). https://doi.org/10.1038/180551a0
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DOI: https://doi.org/10.1038/180551a0
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