Abstract
A PRELIMINARY study has been made of the rate of incorporation of phosphorus-32 into the chromosomes and associated structures in the salivary gland of a Chironomid, Metriocnemus hygropetricus, and since there will be no immediate opportunity to carry the investigation further it seems worth while to present the results, which even at the present stage are not without interest. It has been shown by Prof. H. Bauer (who kindly made available some animals whose progeny were used for this study) that the salivary chromosomes of this species exhibit ‘puffs’ or Balbiani rings of various kinds. Larvæ were grown on medium containing phosphorus-32 (as phosphate) for periods of 2–24 hr., and the incorporation was studied in autoradiographs of squashes and sections, which were stained with methyl-green-pyronin and in some cases extracted with ribonuclease or hydrochloric acid.
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GROSS, J. Incorporation of Phosphorus-32 into Salivary-type Chromosomes which exhibit ‘Puffs’. Nature 180, 440 (1957). https://doi.org/10.1038/180440a0
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DOI: https://doi.org/10.1038/180440a0
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