Abstract
HEATED cellulase of Myrothecium verrucaria 1 degrades the amyloid of Tamarindus indica to a product which by means of paper chromatography (applying butanol/pyridine/water/benzene (5 : 3.2 : 3 : 0.45) as a solvent and developing for a week) appears to contain at least eight oligosaccharides, three of them occurring as the main products. These three oligosaccharides, A, B and C, could be shown to be a hepta-, an octa- and a nona-saccharide respectively, and are composed of 4 glucose residues, 3 xylose residues and 0, 1 and 2 galactose residues respectively. They are liberated in a molar ratio of roughly 2 : 5 : 6. The three oligosaccharides constitute a homologous series; this was confirmed by the facts that: (1) the values of log R F/(1 − R F) lie on a straight line when plotted against degree of polymerization1, and (2) the values of [M]n/n, when plotted against (n − 1)/n, lie on a straight line as well2.
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References
Kooiman, P., and Roelofsen, P. A., Enzymologia, 16, 237 (1953).
French, D., and Wild, G. M., J. Amer. Chem. Soc., 75, 2612 (1953).
French, D., “Adv. Carbohydrate Chem.”, 9, 160 (1954).
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KOOIMAN, P. Partial Enzymic Degradation of Tamarindus-Amyloid. Nature 180, 201 (1957). https://doi.org/10.1038/180201a0
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DOI: https://doi.org/10.1038/180201a0
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