Letter | Published:

Modified Method of Carbon Marking

Naturevolume 180page1415 (1957) | Download Citation



IN the field of experimental embryology, carbon marking has been widely used for tracing the movements of cells1. By this method blood carbon particles adhering to the end of a needle are deposited among the cells the migrations of which are to be studied. When experiments are carried out in ovo, the needle, before it reaches the embryo, must first pass through the overlying layer of saline, albumen, etc., and the surface-tension effects here, as the needle reaches the surface of this fluid, cause the removal of all the particles not adherent to its very tip. To minimize this effect, it is essential that the needle be held perpendicular, and that a quick stabbing movement be used.

Access optionsAccess options

Rent or Buy article

Get time limited or full article access on ReadCube.


All prices are NET prices.


  1. 1

    Spratt, N. T., J. Exp. Biol., 103, 259 (1946).

Download references

Author information


  1. Dept. of Anatomy, Middlesex Hospital Medical School, London, W.1.

    • P. H. S. SILVER


  1. Search for P. H. S. SILVER in:

About this article

Publication history

Issue Date




By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.