Letter | Published:

Isolation of Intact Liver Cells

Naturevolume 180pages12831284 (1957) | Download Citation



LIVER cells have been isolated by various methods, such as by forcing the tissue through stainless steel sieves1, by tryptic digestion2 and by incubation in acid solutions3. Anderson4 obtained a high yield of cells by perfusion of liver with calcium-free solutions containing chelating agents (such as ethylenediamine tetraacetate (versene) or citrate) and subsequent dispersion.

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  1. 1

    Kaltenbach, J. P., Exp. Cell Res., 7, 568 (1954).

  2. 2

    Dulbecco, R., and Vogt, M., J. Exp. Med., 99, 167 (1954).

  3. 3

    Longmuir, I. S., and ap Rees, W., Nature, 177, 997 (1956).

  4. 4

    Anderson, N. G., Science, 117, 627 (1953).

  5. 5

    Branster, M. V., and Morton, R. K., Aust. J. Sci., 19, 72 (1956).

  6. 6

    Kamphausen, H., and Morton, R. K., Biochem. J., 63, 647 (1956).

  7. 7

    Novikoff, A. B., J. Biochem. Biophys. Cyt., 2, 65 (1956).

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    • R. K. MORTON

    Present address: Department of Agricultural Chemistry, The Waite Institute, University of Adelaide,


  1. Department of Biochemistry, University of Melbourne

    •  & R. K. MORTON


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